Objective To investigate whether the single or combined treatments with artemisinin and quercetin can improve. Methods Cell viability was measured, and then the improvement effects on SARS CoV-2 S protein-mediated cytokine storm by artemisinin or quercetin alone or in combination were detected. Results Under the stimulation of SARS CoV-2 S protein, artemisinin or quercetin alone or in combination could significantly alleviate the Cytokine storm by inhibiting the over activation of NFKB. Conclusion Both artemisinin and quercetin alone and in combination can effectively inhibit SARS CoV-2 S protein mediated-cytokine storm.