Objective To investigate the positive infection of human papilloma virus(HPV) among females in 11 group farms in Kashgar, Xinjiang, and to provide reference for HIV prevention and control strategies in ethnic minority areas. Methods From March to June 2020, 5 199 females cervical epithelial exfoliated cell specimens were selected, and 14 HPV subtypes were detected by gene amplification and flow-through hybridization techniques. Results A total of 554 HPV positive samples were detected, with a positive rate of 10.66%, of which single infection accounted for 5.04% and multiple infections accounted for 1.90%. The single infection rate was higher than the multiple infection. The HPV total infection rate and HPV-16/18 infection rate in Uyghur women were significantly higher than those in Han women. The HPV detection rate was the highest in the age group of 30~39 years, followed by 60-69 years old, with detection rates of 13.61% and 10.36%, respectively. Conclusion The prevalence of HPV infection among middle-aged and elderly women in the 11 group farms in Kashgar, Xinjiang is relatively high. Uyghur women are more susceptible to HPV virus than Han women, and single type, HPV-16 and HPV-18 genotypes are more common. Vaccinations should include the two HPV subtypes.