Objective To investigate the content changes of isoorientin in different producing areas, parts, growth period, and harvest periods, so to provide reference for the Campylotropis trigonoclada (Franch.) Schindl. Methods The content of isoorientin Campylotropis trigonoclada (Franch.) Schindl in three major producing areas of Yunnan Dali, Chuxiong and Yuxi, as well as in different parts, growth years and harvest periods of Yuxi were determined by HPLC. Results There are differences in the content of isoorientin in the Campylotropis trigonoclada (Franch.) Schindl from different producing areas. Among them, the content of isoorientin produced in Yuxi Campylotropis trigonoclada (Franch.) Schindl is 190μg/g, which is better than Dali and Chuxiong. There are significant differences in the content of isoorientin in different parts of the Campylotropis trigonoclada (Franch.) Schindl The content of isoorientin is the highest in flowers, followed by leaves, branches, main branches and roots. From 1-year-old to 3-year-old, the change trend of isoorientin content in the Campylotropis trigonoclada (Franch.) Schindl is not obvious; from early July to the beginning of November, the content of isoorientin in the Campylotropis trigonoclada (Franch.) Schindl increased gradually. Conclusions The content changes of isoorientin in different producing areas, parts, growth years and harvest period Campylotropis trigonoclada (Franch.) Schindl were preliminarily revealed, which provided reference for the quality control research and popularization and application of the publication.