The treatment of diseases in traditional Chinese medicine emphasizes regulating shen, which plays an important role in the process of treating diseases and nourishing the body. In the treatment of various diseases with "shen injury" as the main pathogenesis, Professor Yu Tianyuan emphasizes that doctors and patients should follow the four key points:" regulating shen in a steady state, keeping shen to regulate the shen, regulating shen without disturbing shen, and regulating shen with speech induction". Professor Yu has integrated this concept into the clinical regulating shen by acupuncture and tuina, which is not only the inheritance of traditional Chinese medicine classics, but also a clinical innovation. Acupuncture regulating shen emphasizes the use of beishu plus geshu and the four acupoints on the head and face combined with syndrome differentiation to regulate shen. Tuina emphasizes the application of corresponding techniques in the order of relaxing to calm down shen, regulating shen through meridian, regulating shen through dredging qi, and nourishing shen through warm manipulation. This article summarizes Professor Yu's experience in regulating shen.