Objective: A specific HPLC method was developed for the measurement of Loureirin A in Wudidan Capsules .Methods: The sample was analyzed on Agilent ZORBAX XDB C18(250mm×46mm,5μm）column with mobile phase consist of acetic acid（1→90）- acetonitrile （63∶37）at flow rate of 10ml.min-1 and the detection wavelength was 260nm. Results:The calibration curves of Loureirin A was in good linearity over the ranges of 658ug·mL-1 658ug·mL-1，r was 099999，and the average recoveries（n＝6）were 993% and RSD was 145% 。Conclusion:The method is simple,rapid and accurate and can be used for quality control of Wudidan Capsules.