Objective:To study chemical constituents of cultivated Gentiana rigescen and to appraise the qualities of cultivated species by means of comparison on chemical substance with that of the wild species. Method:The chemical constituents were isolated by chromatography and their structures were identified by spectroscopic analysis. The comparison on chemical substance of cultivated and wild Gentiana rigescens was carried out with TLC and HPLC analysis. Results:Nineteen compounds,including main component gentiopicroside,were isolated and identified from the cultivated Gentiana rigescens. The chromatogram of TLC and HLPC analysis of cultivated Gentiana rigescens showed almost same spots or peaks as those of wild species. Their contents of gentiopicroside were 471%(wild species)and 432%(cultivated species),respectively. Conclusion:The chemical constituents of cultivated Gentiana rigescens was reported for the first time. The cultivated Gentiana rigescens was identical in chemical character with the wild species, and the contents of principal bioactive compound gentiopicroside in both of cultivated and wild species were more than the legal standard in China Pharmacopoeia. Planting of Gentiana rigescens was successful and this agricultural technology should be extended.