Objective:To isolate the chemical constituents from the stem bark of Dysoxylum binectariferum and identify their structures. Methods:The powdered stem bark of D.binectariferum were extracted with 95% ethanol and purified by silica gel,RP-C18 and Sephadex LH-20 column chromatograph and HPLC.The chemical structures were elucidated on the basis of spectroscopic analysis.Results:Five compounds were isolated from the stem bark of D.binectariferum including three Sesquiterpenes.The five compounds were identified as 10β,15-Hydroxy-T-muurolol(1),10β,15-Hydroxy-α-cadinol(2),10α,15- Hydroxy-α-cadinol(3),Isoscopoletin(4),3,4-Dimethoxybenzyl alcohol(5),respectively.Conclusion:Compounds 1,2,4,5 are isolated from the genus of Dysoxylum for the first time.