Objective：To study the dissociation constant and oil/water partition coefficient of scutellarein by pH-chromatography. Methods：The dissociation constant of scutellarein is calculated respectively from the retention factors-pH of mobile phase in the pH-chromatography；A oscillate method was established to determine n-octanol/water partition coefficient of scutellarein . Results：The curve of pH-chromatography showed to be suitable for Logistic fit； The pH 30-80 of buffer solution had no influence on the distribution coefficient of scutellarein. Conclusions： pH-chromatography can be used to determine the dissociation constant of scutellarein accurately；The oscillate method can be used to determine the oil/water partition coefficient of scutellarein.