Objective:To investigate the pharmacokinetics and the relative bioavailability of breviscapine pellet capsules in rats.Methods:The cross-over design (two periods) was employed.The pharmacokinetics parameters were compared after rats were administrated a single dose of the oral breviscapine pellet capsules and breviscapine tablets.Resutlts:The main pharmacokinetics parameters of breviscapine pellet capsules and breviscapine tablets as follows: Tpeak were 550±105h and 417±041h；t1/2ke were 297±017h and 204±074 h；Cmax were 150±052μg/mL and 048±018μg/mL；AUC0~t were 609±080μg*h/ml and 200±040 μg*h/mL,respectively. The difference was significant between breviscapine pellet capsules and breviscapine tablets in Tpeak and t1/2ke（P<005）and the bioavailability of breviscapine pellet capsules relative to breviscapine tablets was 30392%.Conclusion:The relative bioavailability of breviscapine pellet capsules was higher than that of breviscapine tablets.