objective:To further research the incense-fumigating oil of R&F,based on the clinical experienced prescription of professor XiongLei and modern pharmacology research.The extra-corporeal bacteriostasis of the incense-fumigating oil of Rhizoma atractylodis & Folium artemisiae argyi,and compare the extra-corporeal bacteriostasis of mixed Volatile Oil and single unmixed Volatile Oil.Methods:to detect the extra-corporeal bacteriostasis of the Incense-fumigating Oil of Rhizoma atractylodis & Folium artemisia argyi and single unmixed volatile oil to different experimental bacteria by the methods of punching,and to figure out its dose-effect relationship of extra-corporeal bacteriostasis respectively by using Levofloxacin Hydrochloride and Fluconazole as positive control.Results:The Incense-fumigating Oil of Rhizoma atractylodis & Folium artemisiae argyi has extra-corporeal bacteriostasis on such bacteria as Staphylococcus aureus, beta streptococcus, candida albicans.Furthermore,its effect,within a certain degree of dose,can boost up with the increasing of dose.However,there is no avail on pseudomonas aeruginosa.Staphylococcus aureus,beta streptococcus,candida albicans are sensitive to single unmixed Volatile oil which in the experiment,Pseudomonas aeruginosa just sensitive to the Volatile oil of Zanthoxylum bungeanum Maxim.