岩白菜中岩白菜素的纯化及提取工艺研究
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(云南中医学院,云南昆明650500)

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R241

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Study on the Purification and Extraction Technology of Bergenin from the Bergama purpurascens(Hook.f.et Thoms.) Engl
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Yunnan University of TCM,Kunming Yunnan 650500,China

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    [摘要]目的:优选岩白菜中岩白菜素最佳提取工艺并考察其纯化方法。方法:用紫外-可见分光光度计测定岩白菜素的含量为指标,考察了影响大孔吸附树脂纯化及醇提工艺的主要因素。结果:确定岩白菜的最佳提取工艺条件为10倍量50%的乙醇,回流提取3次,每次0.5h,最佳的纯化条件为每克D-101树脂(干重)最大上样量2.5g原生药材,上样静置吸附30min后用6倍柱床体积20%的乙醇洗脱。结论:在该提取及纯化的最佳参数组合下,岩白菜素的含量达14.023%,结果可靠。

    Abstract:

    Objective: To optimize the best Extraction process and to investigate its Purification Technology of Bergenin from the Bergama purpurascens(Hook.f.et Thoms.)Engl.Methods:To determine Bergenin content with UV-visible spectrophotometer as an indicator to study the main effect factors of influencing macro-reticular resin purification and ethanol extract technology.Results:Having determined the optimal extraction process of Bergama purpurascens(Hook.f.et Thoms.)Engl.,which include extraction Bergama purpurascens(Hook.f.et Thoms.) Engl.with tenfold 50% ethanol three times,0.5 hour per time, and best purification condition is that loading 2.5 grams net raw medicinal materials at most into D-101 resin each gram and absorb 30mins and the procedure of elution is 6 times 20% ethanol the volume of colume.Conclusion:the content of Bergenin reached 14.023%,which based on The best parameters of purification and extraction,the results are reliable.

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