Objective: Chemical composition of Root-bark of Dense fruit Pittany screen extraction of optimum conditions. Methods: Using high-performance liquid chromatography with UV detection, the relative content of multi-component as evaluation index, by the orthogonal experimental design, studying the concentration of ethanol, ethanol, reflux time and numbers effect chemical characteristics of Root-bark of dense fruit Pittany. Results: The optimum reflux extracting condition obtained: adding12folds of 75% alcohol, extracting once, Every15h.Conclusion: The study of method evaluate the overall hydro-chemical characteristics of traditional Chinese medicine, for evaluation of extraction process for reference.