Objective: To constitute the Pharmacognosy research method about three cultured yews, in order to provide a data basis for deeper exploitation.Methods: Combined botanical identification, key of crude drug characters tissue transections, powder characteristics and TCL to study on pharmacognosy characters of these three yews. Result: The transections of leaves show that there are differences on the shape, size and rang of the epidermal cells, and the structure of leaf midvein is various, the calcium oxalate sand crystal and pigment are various more or less.The characters of stem transections are the differences on the size and rang of the phloem cells, the inclusions in xylem ray cells are only found from Taxus yunnanensis.The sclereids contain crystals , calcium oxalate sand crystals, the number, size, shape of resin ducts can be considered as the characters of powder. The difference between colors and locations of the TCL spots can be used as the identification characters too.Conclusion: This study can be taken as the references for deeper research and identification of these three cultured yews crud drugs.