Objective:To observe the effects of major components in Yunnan Baiyao on the Angiogenesis during the process of experimental rabbit fracture healing.Methods:100 New Zealand rabbits were employed and randomly divided into 5 groups in this experiment. After the fracture model of both radius was established by operation , every medical gelatin sponge which contains number1,2,3,4,6 Yunnan Baiyao component be put into all left bone defect of radius separately as 1,2,3,4,6 Yunnan Baiyao group, and put an medical gelatin sponge which contains nothing into all the right defect as matching control group.To observe the effect of Yunnan Baiyao on the expression of VEGF and CD31 at different intervals was observed in the course of bone healing.Results:The expression levels of VEGF and CD31 in number1-6 Yunnan Baiyao group was surpassed to that of control group at the early and middle stage of fracture healing and the highest levels of expression was the 2 weeks after operation，and the lowest was 12 weeks after operation.Conclusion：Yunnan Baiyao can enhance the expression of VEGF and CD31 in the callus tissue，accelerate the vascularization at the broken ends of fractured bone，improve the blood circulation of the fractured extremity，thus it promote the healing of fractured bone.