Objective: Select the best condition for extraction process of total flavonoids from Radix Astragali. Method: Regard the total extract yield and the yield of total flavonoids as indicators, total flavonoids extraction conditions were optimized by using orthogonal test method. Four factors were examined by three levels: the amount of solvent(A), ethanol concentration(B), extraction time(C),extraction frequency(D).Results: The best extraction process of total flavonoids from Radix Astragali by ethanol is A2B2C1D2,that is,it was extracted with 10 times the amount of 70% ethanol two times, 60min every time. Conclusion: The method can provide a stable basis for extraction process of total flavonoids from Radix Astragali.