Objective:The contents of 2,3,5,4'-tetrahydroxy stilbene-O-β-D-glucoside(TSG)in 21 samples of Polygoni multiflori Caulis from different districts of Yunnan Province.Methods:The HPLC was applied for the determination of the contents of TSG in samples.Results:The contents of TSG were in the range of 0%-1.508%.Conclusion:The results showed that the contents of the samples from different districts in Yunnan Province were quiet different, and part of them were not up to the standard of Pharmacopoeia of the People's Republic of China,which providing the basic data of Polygoni multiflori Caulis from Yunnan Province.And the quality control of Polygoni multiflori Caulis should be strengthened.