Objective:To explore the factors influencing the seed germination and radicle growth,and provide technical guidance for seed breeding and reproduction of Paris polyphylla.Smith var.yunnanensis(Franch.)Hand.-Mazz.Methods:To study the effect of seed coat,temperature,GA₃,humidity,different provenance on the seed germination and radicle growth of Paris polyphylla.Smith var.yunnanensis(Franch.)Hand.-Mazz.Results:The seed coat and Low temperature has a obvious inhibitory effect for the seed germination and radicle growth of Paris polyphylla.Smith var.yunnanensis(Franch.)Hand.-Mazz; GA₃ can break the inhibition on seed germination and radicle growth caused by low temperature; The seed has a broad range of humidity for seed germination and radicle growth; The seeds from different provenance,largely because of the different maturity,get a completely differences on seed germination and radicle growth.Conclusion:in the seed breeding process for Paris polyphylla yunnanensis(Franch.)Hand.-Mazz,We should select good maturity seeds for the seed breeding,Both removing the seed coat and GA₃ treatment can improve the seed germination and radicle growth rate.