Objective: To investigate the chemical constituents of Stephania epigaea. Methods: the powered tuber were extracted with 2% H2SO4-H2O and purified by silica gel and Sephadex LH-20 column chromatograph. The chemical structures were elucidated on the basis of spectral analysis and physico-chemical evidence. Results: ten alkaloid compounds were obtained from the tuber of S.epigaea, they are characterized as stephanine (1), (-)-cassythicine (2), dicentrine (3), isocorydine (4), sinoacutine (5), sinomenine (6), cephamonine (7), stepholidine (8), palmatine (9) and tetrahydropalmatine (10). Among them, compounds 7-9 were obetained for the first time from S. epigaea.