乌头赤石脂丸对寒凝胸痹大鼠损伤心肌和血液粘度的作用研究
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南京中医药大学第一临床医学院,江苏南京 210046

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R285.5

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基金项目:南京中医药大学青年基金项目(NO:09XZR06) 收稿日期:2012—04—01


Study on Effect of WutouChishizhi Pill on Myocardial Injury and Blood Viscosity in Rats with Chest Bi
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The 1st Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing Jiangsu 210046,China

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    摘要:

    目的:研究乌头赤石脂丸对寒凝胸痹大鼠损伤心肌的保护作用和血液粘度影响。方法:冰水游泳14d加末日腹腔注射垂体后叶素20U/kg造成大鼠寒凝胸痹模型。注射垂体后叶素30min内描记Ⅱ导联心电图变化; 24h后,颈总动脉采血,检测血中CK、LDH水平和血液粘稠度,并取下心脏,观察心室形态学。结果:与模型组相比,预先给予复方丹参滴丸组及乌头赤石脂丸中、高剂量组能减缓Ⅱ导联J点上抬(P<0.05),降低血清CK、LDH含量和血液粘稠度(P<0.05),减轻心室肌组织受损程度。结论:乌头赤石脂丸能减轻寒凝胸痹大鼠的缺血心肌损伤,对缺血心肌有保护作用,并降低血液的粘稠度,这可能是其保护作用的机制。

    Abstract:

    Objective:To observe the effect of Wutou Chishizhi pill on myocardial injury and blood viscosity in rats with chest Bi caused by cold and pituitrin. Methods: The chest Bi rat model was induced by rat swimming in ice water for 14 days and pituitrin 20U/kg injected intraperitoneally at the last day.After pituitrin injected, ECG Ⅱ was traced for 30 minutes.24 hours later, blood were collected via carotid artery for assaying the plasma CK、LDH activity and blood viscosity, and the cardiac ventricle were dissected and examined with light microscope. Results:CDDPG、YPMG and YPHG can lower the elevation of J point of ECGⅡ( P<0.01),and obviously decrease the activities of CK、LDH and blood viscosity(P<0.05),and lessen the myocardial injury. Conclusion:Wutou Chishizhi pill has a protective effect on myocardial injury in rats with chest Bi caused by cold and pituitrin. It can lower blood viscosity, and thus, may enhance the cardiac blood supply, which may explain how Yutouchishizhi pill works.

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