Objective:To investigate the Anti-inflammatory effects of orally or topical plus intragastric administration of Yunnanbaiyao tincture in rats. Methods:The anti-inflammatory effect of Yunnanbaiyao tincture was investigated not only on rats model of adjuvant arthritis induced by Freund's complete adjuvant, but also on joint pain caused by uric acid sodium. Results:The Yunnanbaiyao tincture were given by external or intragastric administration could significantly alleviate the primary swelling of on foot subjected to being injected Freund's complete adjuvant, and also could extenuate secondary swelling on contralateral foot. At the same time, it could reduce to the swelling score of the forefoot. In rats model of joint pain caused by uric acid sodium, orally or topical plus intragastric administration of Yunnanbaiyao tincture could significantly inhibit the swelling of rat arthritis,and significantly reduce the gait score about rats. Conclusion:Intragastric or topical administration of Yunnanbaiyao tincture has obvious anti-inflammatory not only on rats model of adjuvant arthritis induced by Freund's complete adjuvant,but also on joint pain caused by uric acid sodium. Topical plus intragastric administration of Yunnanbaiyao tincture shows a certain synergistic effects in some pharmacodynamic trials. Moreover,topical administration of Yunnanbaiyao tincture has positive effects on microcirculation in mice.