Objective:To establish a method for the determination of 12 organophosphorus pesticides in Panax notoginseng by gas chromatography,which collected from different cultivate areas.Methods:The organophosphorus pesticides were determined according to the approaches listed in the chinese pharmacopeoa published in 2010.Results:There are three pesticides(diazinon,parathion and malathion)were found in tested samples.Diazinon was found in Panax notoginseng stems and leaves from Luxi and Mile with a content of 0.13mg/kg.Parathion of 0.03mg/kg was detected in the roots from Wenshan.Malathion was contained in crude materials of notoginseng from Wenshan and Luxi,in which residue was particularly high in stems and leaves from Luxi at 0.58mg/kg.Conclusion:This is a simple and applied method for determine residues of organophosphorus pesticides.It could be uses not only on herbal materials but also other cultivates plants.The analysis results showed that pesticide residues become a haunting problem in cultivated Panax notoginseng.