Objective: To establish the method for determination of two kinds of heavy metals( As,Hg) in Glycyrrhiza of Sang Chan Zhi Ke Fang by atomic fluorescence spectrometry(AFS). Methods: Microwave digestion and determination by AFS. Results:The content of As is 03857mg/Kg; The content of Hg is 58274μg/Kg Accord with the chinese pharmacopoeia under the provisions of Glycyrrhiza. The correlative coefficient of the calibration curves was over 09997, the recovery was 995%~1028%, RSD≤22%. Conclusion: The method is quick ,convenient ,acquired accurate and highly sensitive.