Objective：Testing the physiological and biochemical parameters in mice, to evaluate the nature of unprocessed Rhizoma Pinelliae (RP) and Rhizoma Pinelliae With Bamboo Vinegar Processed(RP-BVP). Methods：Measured weight, water intake, food intake, body temperature, oxygen consumption, total protein content of liver tissue, Na-K-ATPase activity, liver coefficient of mice after giving the water decocted solution of RP and RP-BVP intragestrically 11days. Results：Compared with control group, weight, food intake, liver coefficient had no significant difference in RP and RP-BVP group; Compared with control group, water intake, body temperature, oxygen consumption, Na-K-ATPase activity, total protein content of liver tissue increased in RP group; oxygen consumption, the liver tissue total protein content, Na-K-ATPase activity reduced in RP-BVP group. Conclusion：water intake, body temperature, oxygen consumption, Na-K-ATPase activity, total protein content of liver tissue can be used to evaluate the cold and heat nature of RP and RP-BVP.