Objective: It is to provide objective and theoretical basis in treating NAFLD based on the traditional Chinese medical syndrome differentiation of NAFLD population and the exploration of the relationship between the differentiation, body mass index and blood fat. Methods: Among check-up crowd of Shanghai Shuguang hospital affiliated with Shanghai University of Traditional Chinese Medicine (TCM) from Jan to Jun 2011, 602 cases meet the standards of NAFLD diagnosis, for the study. Relatiohnship between different types, body mass indes and blood fat was analyzed. Result: 1) based on the TCM syndrome distribution of NAFLD population, liver stagnation and spleen deficiency syndrome becomes the most common and liver and kidney deficiency syndrome at least 2) Obesity rate of phlegm and blood type and damp type is the highest, statistically significant differences between their group (P＜005). 3) From a statistical point of view of fat parameters, TG gets different degrees of increase in each group. To the phlegm and blood type, it increases significantly. TC also increased in varying degrees, but significantly increased with liver and kidney deficiency type. LDL-C rise is mainly concentrated in the liver depression and spleen group. There’s no significant difference of HDL-C among different groups. Result:NAFLD syndrome differentiation is closely related to body mass index, TG, TC and LDL-C. This objective can provide theoretical basis in Treating NAFLD.