Objective To explore the action of the Jian Pi Shen Shi Decoction lowers uric acid hyperuricemia in rat model and the regulation of urate transporter protein （ UAT）.To reveal the biological characteristics and the law of treatment of gout with “Jian Pi Shen Shi， Phlegm meridians”. Methods 48 rats were divided into 6 groups， the control group， the model group， the positive group（benzbromarone）， Jian Pi Shen Shi Decoction low dose group， Jian Pi Shen Shi Decoction middle dose group， and Jian Pi Shen Shi Decoction high dose group. All the groups except the control group were fed with fodder yeast plus adenine to get the hyperuricemia rat model. Then after 14 days， test trioxypurine， creatinine， and urea nitrogen of the drenched rats and analysis the expression UAT protein in HK-2 tubular epithelial cells effected by the containing serum of Jian Pi Shen Shi Decoction with Western blot . Results Three dose groups of Jian Pi Shen Shi Decoction can effectively lower trioxypurine of the hyperuricemia rat model（P<0.05）， and also creatinine and urea nitrogen（P<0.05，P<0.01）. The containing serum of Jian Pi Shen Shi Decoction significantly raised the expression of urate transporter（UAT）. Conclusion Jian Pi Shen Shi Decoction can effectively protect the renal function and lower the uric acid， the mechanism of action may be related to with raising the expression of urate transporter.