Objective To clarify influence on brain cognitive functional connection of acupuncture at DU26 based on cognitive impairment. Methods Needling at DU26 was performed in six subjects. 6 min of scanning at rest were adopted before and after acupuncture at different acupoints. Changes of brain functional connection were observed. Results During needle retaining of acupucunture DU26， trends toward decrease in the connections of the IPL， left DLPFC， but toward increases in the connections of the right DLPFC with left DLPFC， right IPL， right frontal cortex， connections of the right aPFC with left aPFC， connections of the left IPL and dACC/msFC， VMPFC with TPJ， al/fo， left midtemporal. There are no fainting on acupuncture， sticking of the needle， bending of the needle， hematoma and other bad effects. Conclusion During needle retaining of acupucunture DU26， brain areas related to cognitive and emotional were activated.