Alsophila spinulosa（Hook.）Tryon is one of the only existing woody ferns which is extremely scarced. It protected by many countries as an endangered plant. In this paper，the shape properties，microscopical feature，physical and chemical appraise of Alsophila spinulosa were studied by pharmacognosy method，such as their physicochemical characteristics were identified by using thin layer chromatography（TLC）. Thus，it can be taken as the establishment of quality standard，pharmacognostic studies and the further utilization of the leaves of Alsophila spinulosa（Hook.） Tryon through the study on their pharmacognostic characteristics.