Objective To determine the content of flavonoids and polyphenols in the three walnut kernel pellicle from Yunnan different areas of. Methods With the walnut kernel pellicle as raw material，flavonoids in walnut kernel pellicle by colorimetric method with rutin as control，polyphenols in the walnut seed capsule was determined by Folin-Ciocaileu colorimetric method with gallic acid as the standard. Results Rutin in 0~0.1mg/mL，gallic acid in 0～50μg/mL range，absorption value showed a good linear relationship with concentration. The flavonoids in the Yunnan Maguo walnut，Midian walnut and Caoguo walnut kernel pellicle are 15.48%，17.33% and 15.23%，respectively，the phenolic compounds were 57.58%，31.25% and 24.01%. Conclusion The three kinds of Yunnan walnut kernel pellicle contain abundant flavonoids and polyphenolic compoundse. And polyphenols in the Maguo walnut kernel pellicle is as high as 57.58%，with the potential of research and development.