Objective To research the air disinfection effects of the incense-fumigating oil of Rhizoma atractylodis & Folium artemisiae argyi（R&F），in order to use a safer and efficient disinfection method for the pediatric wards，as well as to provide experimental basis. Methods To detect the air disinfection effect of the incense-fumigating oil of R&F by air disinfection experiment and space sterilization experiment，in where Pediatric inpatient department general ward，therapeutic room，outpatient clinic and special metal cabinet，and to explore its mechanism of action. Results The air disinfection effect of the incense-fumigating oil of R&F is better than vinegar smoked and ultraviolet irradiation. The incense-fumigating oil of R&F has space sterilization effect on such bacteria as Staphyococcus aureus，Escherichia coli，Beta streptococcus，Candida albicans，and just has certain inhibition effect for the Pseudomonas aeruginosa. Conclusion The effectincense-fumigating oil of R&F，in order to further research and development of new traditional Chinese medicine air disinfectant provides the partly experimental basis.