Objective To evaluate regulation of the AQP1，AQP3 in bladder post oral administration of a diuressis-reemoving dampness chinese medicine，sclerotia of P. umbellate aqueous extract in urinary bladder cancer rats. Methods The sclerotia of P. umbellate aqueous extract given Fisher-344 rats induced by N-butyl-N-（4-hydroxybutyl）nitrosamine（BBN）combined saccharin，then the regulation of AQP1，AQP3 measured with immunohistochemical and RT-PCR. Results The AQP1 and AQP3 mRNA relative expressions in bladder carcinogenesis rat bladder post 250mg/kg，500mg/kg sclerotia of P. umbellate aqueous extract doses treatment were down-regulated compared to BBN group. Conclusion Inhibition of bladder carcinogenesis by sclerotia of P. umbellate aqueous extract associated with regulation of AQP1 and AQP3 in bladder.