Objective To compare the three matrix transdermal effects of four medicinal ingredients （camphor，menthol，borneol and methyl salicylate） of The Yunnan Baiyao rubber cream，Babu Plaster and hot melt adhesive by Gas Chromatography Method. Methods Establishment of the slow mouse skin penetration model，using the percutaneous absorption instrument，samples were collected to detect the content at 1，3，5，8 hour. Results Under the premise of the same drug loading，Yunnan Baiyao Babu Plaster were better than the rubber cream and hot melt adhesive significantly in transdermal effect；little difference between the rubber cream and hot melt adhesive. Conclusion Yunnan Baiyao Babu Plaster meet the demand for clinical treatment in transdermal effect more easily，this new type of adhesive matrix can ensure the production quality and expected efficacy of Yunnan Baiyao cream products，and explore the international market for the product in the future.