Objective The Pollen morphology of 15 Aconitum species in Yunnan Province was observed under scanning electron microscope，can not only facilitate the identification of species in this genus，but also help to guarantee the safe usage of Radix Aconiti herbs. Methods Optical and electron microscopes were exploited to observe the pollen of 15 Acotium species and then comparison were made among their morphology. Results The result showed that the pollen grains in genus Aconitum have 3 germpores，obvious furrow membrane with granular lumps ornodules. Tectum have concavities and spiny or smooth ornamentations. The shapes of pollen in polar view were generally three-lobed circular or blunt trianglar，and occasionally striangular circular. The shapes of pollen in equatorial view could be categorized into three types：nearly round、circular wide oval or nearly oval. Conclusion Our research provides new data on pollen morphology of Aconitum species，which，in combination with exsiting data.