Objective To observe the treatment effects on heart rate variability of electro-acupuncture at different primary point with acute myocardial ischemia rabbits. To investigate the specificity effects of adjusting cardiac function after electro-acupuncturing（EA） on different primary acupoints. Methods 56 healthy rabbits are selected， 8 are chosen randomly as normal group. The odd rabbits were developed acute myocardial ischemia models by intravenous administration posterior pituitrin into femoral vein， and AMI model rabbits were randomly divided into model group， EA on HT7 group， EA on KI3 group， EA on PC7 group， EA on LR3 group and EA on nonpoint group， 8 in each group. The heart rate variability were analyzed by BIOPAC system and then analysis the signals with HRV&BRS 2.00 software. Results The HR， RRI， TV， LF/HF of EA on HT7 group， PC7 group and LR3 group have significant differences comparing with model group（P<0.05）， while the HR， RRI， TV， LF/HF of EA on KI3 group and EA on nonpoint group after EA have no significant differences comparing with model group（P>0.05）. Conclusion HT7 and PC7 have the best effect on the adjusting heart rate variability of acute myocardial ischemia rabbits， while LR3 has certain adjustment effect and KI 3 has not obvious effect.