Objective To investigate the effects on expression of ERK and p38MAPK in UC rats with syndrome of dampness stagnancy due to spleen deficiency that treated with Shenlinbaizhu powder. Methods 40 rats were divided into normal control group， model control group， Shenlinbaizhu group and sulfasalazine group， 10 in each group. The model rats were induced by environment and diet intervention combine with composite TNBS and ethanol. The expression changes of ERK and p38MAPK in rats were detected by immunohistochemistry. Results The expression of ERK and p38MAPK in the model group was more than in normal group（P<0. 05）. The expression of ERK and p38MAPK in sulfasalazine group and Shenlinbaizhu group significantly decreased and shown significant difference compared with those of model group（P<0. 05）. There was significant difference between sulfasalazine group and Shenlinbaizhu group on the expression of ERK and p38MAPK（P<0. 05）. Conclusion The expression of ERK， p38MAPK in the UC rats with syndrome of dampness stagnancy due to spleen deficiency was increased， and Shenlinbaizhu powder could significantly improve the expression of ERK， p38MAPK in the colon tissue of UC rats.