Objective To study the influence of Curcumin on the expressions of TLR4 and TNF-a、IL-6 、IL-8 release in pulmonary macrophage. Methods The experiments were performed in culture of LPS induced pulmonary macrophage in vitro， the TNF-a、IL-6 、IL-8 release was detected with ELISA and the expressions of TLR4 was detected with RT-PCR and western blot. Results Pulmonary macrophage was stimulated with LPS and the expressions of TLR4 and TNF-a 、IL-6 、IL-8 release was increased（P<0.01）， but it was inhibited by Curcumin（P<0.05）， and dose dependence（P<0.05）. Conclusion The mechanism of Curcumin’s antiinflammatory may be reduce cytokine release， by inhibits expressions of TLR4 and via Cross talk toll-like receptor-4 signaling；Curcumin may be used as a kind of anti-infective drug.