Objective To investigate the somatosensory music low-frequency sound waves（16 - 160Hz）influence on the healthy people twelve main and collateral channels acupoints microcirculation， summarize the main and collateral channels resonance frequency. Methods 30 cases of healthy people in the vicinity of twelve main and collateral channels acupoints according to the order of play， somatosensory music low-frequency sound waves， at the same time with laser Doppler flowmetry meridian acupoints of microcirculation， statistical analysis of experimental data. Results Twelve main and collateral channels acupoints microcirculation frequency influence the most significant respectively， Shangqiu：A0#（29.14Hz）， JieXi：A1#（58.27Hz）， in the letter：D1（36.71Hz）， Yo Suke（73.42Hz）：D2， the drainage：C1（32.70Hz）， Yangxi：C2（65.41Hz）， Fu Liu：G1（49.00Hz）， KunLun：G2（98.00Hz）， the path：F1（43.65Hz）， Yanggu：F2（87.31Hz）， the：A1 （55.00Hz）， channel：A2（110.00Hz）. Conclusion The main and collateral channels and acupoints of different selective absorption characteristics of the low frequency acoustic wave with different frequencies， the specific frequency sound waves can cause resonance of main and collateral channels.