Objective To investigate the effects on expression of NF-κB p65 and correlative inflammatory factors in myocardial ischemia reperfusion injury rats with acupuncture at PC6 and BL15. Methods 60 rats were randomly divided into sham operation group， model control group， acupuncture PC6 and BL15 group （acupuncture observation group） and acupuncture LU9 and BL13 group （acupuncture control group） according to random number table， 15 in each group. The rats of acupuncture observation group were treated by electro-acupuncture at PC6 and BL15 with 1mA， 2Hz， 20min of each stimulus， 1 time a day， a total of 3 days. The rats of acupuncture control group were treated by electro-acupuncture at LU9 and BL13 with methods same as acupuncture observation group. The rats of sham operation group and model control group did not use electro-acupuncture. The myocardial ischemic area， infarction area， contents of IL-1β and IL-10， expression of NF-κB p65 in each group were detected. Results The myocardial ischemic area， infarction area， content of IL-1β and expression of NF-κB p65 in model group significantly increased， the content of IL-10 decreased， which shown significant difference compared with sham operation group （P<0. 05）. The myocardial ischemic area， infarction area， content of IL-1β and expression of NF-κB p65 significantly decreased， content of IL-10 increased compared with model control group and acupuncture control group（P<0. 05）. Conclusion Acupuncture at PC6 and BL15 could significantly decrease the content of IL-1β and expression level of NF-κB p65， and incerase the content of IL-10， which may be one of the mechanism of acupuncture effect in anti-myocardial ischemia.