Objective To observe the effect of rhubarb induced mild moxibustion；explore the mechanism of moxibustion treatment of spleen deficiency syndrome. Methods 40 healthy SD rats were randomly divided into 4 groups， namely the normal saline and mild moxibustion group、the normal saline without moxibustion group， the rhubarb and mild moxibustion group、 the rhubarb without moxibustion group. Water decoction of rhubarb was made by filling the stomach spleen deficiency rat model， observe the general condition of SD rats， determine the level of plasma β-EP endorphin by radio immunoassay. Results compared with the normal saline without moxibustion group， the normal saline and mild moxibustion group’s plasma β-EP levels significantly increased（P<0. 01）； compared with the rhubarb without moxibustion group and the rhubarb group， the rhubarb and mild moxibustion group’s plasma β-EP levels were significantly increased（P<0.01）； compared with the rhubarb group， the rhubarb without moxibustion group’s plasma β-EP level increased（P<0.05）. Conclusions Moxibustion at zusanli、zhongwan point can obviously improve the rat model of spleen deficiency syndrome of spleen deficiency， mild moxibustion has regulatory effect on normal rats and rats with spleen deficiency plasma β-EP levels， the regulation of plasma β-EP levels may be one of the mechanism of Moxibustion in the treatment of spleen deficiency， may also be one of the mechanism of building up normal individuals’ constitution and preventing disease.