Objective To apply Dantong tincture outside to treat rats with diabetic peripheral neuropathy. Methods Synchronized to the tail skin trans-dermal delivery methods. By the reaction time of tail swing of rats recorded by the analgesic instrument can determine the treatment of diabetic peripheral neuropathy by Dantong tincture. Capsaicin ointment acts as positive control， 75% ethanol as negative control， healthy male rats as normal control group. Results Dantong tincture can significantly reduce the pain of diabetic peripheral neuropathy and the response time of tail swing of rats， with capsaicin group produce the same effect of improving nerve conduction velocity in rats. Conclusion Dantong tincture can cure diabetic peripheral neuropathy.