Objective To study the chemical constituents of the roots of seseli mairei Wolff. Methods The chemical constituents have been separated with manifold chromatography methods， and their structures were determined by spectral analysis. Results Nine compounds were isolated and identified as falcarindiol（1）， falcarinol（2）， （8E）-heptadeca-1， 8-dien-4， 6- diyn-3， 10-dinol（3）， stearic acid（4）， bergapten（5）， β-sitosterol（6）， daucosterol（7）， mannitol（8）， sucrose（9）. Conclusion The compounds 1，2，8-9 were isolated from the roots of seseli mairei Wolf. for the first time.