Objective To study the change of endogenous hormone of the Paris polyphylla var in the course of seed germination and provide theory basis for seed breeding of Paris polyphylla. Methods After the seeds were treated by two different conditions including indoors and outdoors， the content of endogenous hormone （GA、IAA and ABA） was mensurated by RP-HPLC. Results After being treated for 120 days， the seed of Paris polyphylla var breed；During germination， the content of GA rose， the content of ABA reduced， the content of IAA remained；The germination level of the seed treated by outdoor conditions was higher than that of the seed treated by indoor conditions. Conclusion ABA and GA have a significant impact on the germination of Paris polyphylla var yunnanensis seeds.