Objective To observe the clinical effects of“strengthening spleen and clearing intestinal treatment ”on intestinal barrier dysfunction in patients after chemotherapy with colorectal cancer， and its mechanism. Methods 82 cases of intestinal barrier dysfunction in patients after chemotherapy with colorectal cancer were divided into three groups， the blank control group in which 26 cases were treated by single chemotherapy， the control group of Bifico in which 26 cases were treated by Bifico， while the treatment group of traditional Chinese medicine were given strengthening spleen and clearing intestinal decoction. Before and after the treatment for two mouths， KPS score， overall accumulate points and single point of TCM symptoms were all aberserved， the blood endotoxin concentration and the ratio of urine lactulose to mannitol（L/M）were also detected. Results Treatment group can raise scores on the quality of life（P<0.05）. The efficiency of treatment group which can improve significantly clinical symptoms of spleen deficiency and damp-heat accumulation in patients is 83. 33%（P<0.05）， which is higher than the other groups. This decoction can significantly improve symptoms of “loose stool， abdomen pain， poor appetite， borborygmus， tiredness， yellow complexion”（P<0.05）. The blank control group and the Bifico control group can make some symptoms aggravating in different degree. It can also produce the same effect with Bifico for “loose stool ”. （P>0.05）Strengthening spleen and clearing intestinal decoction and Bifico can all decrease significantly endotoxin， urine lactulose / mannitol ratio（P<0.05）. Conclusion Strengthening spleen and clearing intestinal decoction can significantly ameliorate intestinal barrier dysfunction in patients with colorectal cancer， improve clinical symptoms of spleen deficiency and damp-heat accumulation