Objective To evaluate the correlation between TCM dialectical type and T lymphocyte subsets， IL-4， IFN-γ in patients with HBeAg-negative chronic hepatitis B. Methods 120 patients with HBeAg-negative CHB were diagnosed with differential Chinese medicine syndrome， and their peripheral blood T lymphocyte subsets（CD3+， CD4+， CD8+ and CD4+/CD8+）， IL-4 and IFN-γ level were determined. Results Compared with those in the normal control group， CD4+ was lower， CD8+was higher， CD4+/CD8+ was decreased significantly（P<0.05 or P<0.01）， IL-4 was significantly elevated（P<0.01） and IFN-γ was significantly lower（P<0.01） in the HBeAg-negative CHB patients. Comparison among the different syndrome types revealed that in damp heat resistance and blood-stasis in meridian， T lymphocyte subsets was statistically different with liver-kidney yin deficiency， spleen-kidney yang deficiency（P<0.05 or P<0.01）. In the damp heat resistance， liver stagnation and spleen deficiency， blood-stasis in meridian， liver-kidney yin deficiency， spleen-kidney yang deficiency sequence， IL-4 rose gradually and IFN-γ decreased gradually， with significant differences between groups（P<0.01 or P<0.05）. Conclusions It is related that the peripheral blood T lymphocyte subsets， IL-4， IFN-γ levels and TCM dialectical type in patients with HBeAg-negative CHB.