Objective To investigate the effect of Gushu granules on Ovariectomized Osteoporotic Model Rats E2、T 、P 、PRL 、FSH 、LH. In order to provide experimental basis for clinical application of Gushu granules and further study. Methods 40 SD rats were randomly divided into 4 groups：model group， blank control group， estrogen group and group of Gushu granules. The osteoporosis model was induced by ovariectomy. Serum levels of sex hormone E2、 T、 P、 PRL、 FSH、 LH change in rads were observed after the rats were treated with Gushu granules and western medicine（estradiol valerate）， femoral osteoporosis pathology and biochemical indicators of change emical indicators of change were catch， too. Results Gushu granules and estradiol valerate could increase the level of E2 and T. Significant difference（P<0. 005）was compared with model group. They ease osteoporosis pathological changes， elevate calcitonin， reduce bone alkaline phosphatase、tumor necrosis factor 、tartaric acid alkaline phosphatase and urine hydroxyproline（P＜0. 01 or P＜0. 05）. Conclusion Gushu granules could treatment and delay osteoporosisimprove. There was no significant difference with estrogen.