Objective To research the influence on cartilage ultrastructure in the rabbit knee osteoarthritis under the electron microscope by the massage manipulation of stretch pull loose. Methods The adult Japanese white rabbits which made model of knee osteoarthritis by the building methods of Videman T were randomly divided into the stretch pull loose manipulation group、the sodium hyaluronate injected group、the model group and the blank group. To observe the change in cartilage ultrastructure of the rabbit knee osteoarthritis under the electron microscope after the treatment. Results The stretch pull loose manipulation has accurate therapeutic for the repair of damaged cartilage cells of rabbit knee osteoarthritis and it can promote the repair of the surface structure of cartilage. Conclusion The stretch pull loose manipulation can promote the repair of injury of knee osteoarthritis chondrocytes and cartilage surface structure， and it is the objective basis for the treatment of knee osteoarthritis.