Objective To observe the protective effects of Shenmai injection in Zusanli acupoint injection on rats systemic inflammatory response syndrome inflammatory factor TNF-α. Methods To establish a comprehensive model of systemic inflammatory response syndrome in rats. Forty SD rats were randomly divided into four groups（n=10）， including Vacuity-contrast group（VC group）、 Acupuncture in Zusanli group（AZ group）、 Shenmai injection in Zusanli acupoint injection group（SZ group） and Physiological saline Zusanli point injection group（PZ group ）. To observed the general condition of rats when modeling success after 1h. After 5D treatment the rats were sacrificed， collect samples， detection of blood （white blood cell count， WBC）； arterial blood gas analysis（PCO2， PO2）； serum tumor necrosis factor alpha （TNF-α） level. Results The WBC、 TNF-α levels of the SZ group were significantly lower than the other three group（P<0.05 ）. The PCO2 levels of the SZ group were significantly lower than VC group（P<0.05 ）. The PO2 levels of the SZ group were significantly higher than VC group（P<0.05 ）. Conclusion Shenmai injection in Zusanli acupoint injection for treatment of SIRS in rats may reduce the inflammation response， inhibit inflammatory factors， to protect lung function.