Objective To establish a HPLC method for the determinations of paeoniflorin， baicalin and paeonol in Biejiajian pills. Methods Agilent Zorbax Eclipse XDB C18 column（5μm， 4.6 mm×150 mm）， mobile phase of acetonitrile（A ）and 0. 1% phosphoric acid（B）. Gradient elution， flow rate of 1.0 mL/min， the detection wavelength of 240nm and 274nm， the column temperature was 35℃. Results The linearity ranges of paeoniflorin， baicalin and paeono was 1.41 ~ 14.11μg/mL （r2=0.9999）， 7.03 ~70.32μg/mL（r2=0.9999）， 1.23 ~12.32μg/mL（r2=0.9997） respectively. The average recoveries（n=9）were100.84%， 99.07%， 99.08%. Conclusion The results showed that the method can be used to control quality of Biejiajian pills.