Objective Study to establish a pharmaceutical analysis method for determination of Scutellarin content in Dengzhanxixin mixture（sugar-free）. Methods Using HPLC to determine the optimal method for content determination. Results Use of C18 column and a mobile phase of methanol-tetrahydrofuran-0. 1% phosphoric acid（14∶14∶72）. The flow rate was 1. 0 mL·min-1. The detection wavelength was 335nm. There was a good linear range of 0. 005244 to 0. 8391 μg（r=0. 9998， n=5） for Scutellarin. The minimum detection limit was 3.648μg . The average recoveries were 98. 2％， RSD=1. 71（n=6）. Conclusion The method is convenient， rapid and accurate with good reproducibility and could be used for the quality control of Dengzhanxixin（sugar-free）mixture， effective content of drug quality.