Objective To investigate the effect of QingReXiaoJi on the expression of hypoxia inducing factor 1 alpha（HIF-1α）protein of Tumor Tissue， and to explore the mechanism of anti-tumor angiogenesis. Methods 60 C57BL/6J inbred mice with Lewis lung carcinoma xenograft model were randomly divided into five groups：blank control group（NS group）， positive control group（CTX group） and QingReXiaoJi group divided into high， medium and low dose group（the amount of crude drug were 1.12g/mL， 0.56 g/mL， 0.28g/mL）， 12 rats in each group. The blank control group and QingReXiaoJi high， medium， low dose group were respectively feed with physiological saline， traditional Chinese medicine decoction in 0.2mL/10g， the positive control group was treated with intraperitoneal injection of cyclophosphamide. Using immunohistochemical method and Western blot to detect the expression of HIF-1α protein of Tumor Tissue. Results The MOD of HIF-1α protein in QingReXiaoJi high， medium， low dose group， CTX group were lower than NS group（P<0.05）， the A% of HIF-1α protein in QingReXiaoJi high， low dose group， CTX group were lower than NS group（P<0.05）， and QingReXiaoJi in each dose group showed no difference between MOD and A% （P>0.05）. Conclusion QingReXiaoJi can cut the expression of HIF-1α protein， which may be one of anti-tumor angiogenesis effects.