Objective To determine the equilibrium solubility of Piceatannol 3’-O-β-D-glucopyranoside by UV Spectrophotometry in different media and the octanol-water and octanol in a buffer fluid system apparent oil-water partition coefficient to provide a basis for the development of design Piceatannol 3’-O-β-D-glucopyranoside preparations. Methods Measuring the equilibrium solubility of Piceatannol 3’-O-β-D-glucopyranoside in different medium. Using the shake flask method for the determination of Piceatannol 3’-O-β-D-glucopyranoside apparent oil-water partition coefficient， measuring wavelength of 319nm. Results The equilibrium solubility of Piceatannol 3’-O-β-D-glucopyranoside in water was 5. 77 mg/ml at 37 ℃， and the solubility was 4.53mg/mL in 0.1mol/L HCl， in physiogical saline was 5. 21mg/mL， in a phosphate buffer solution of different pH value， its solubility gradually increased with the increase of pH；Piceatannol 3’-O-β-D-glucopyranoside in n-octanol and water relative to the performance of oil-water partition coefficient， Papp was 3. 12；when pH is during 2 to 8， there is no significantly affected by pH influence performance hydrophilic. Conclusion The equilibrium solubility in water and oil-water partition coefficient of Piceatannol 3’-O-β-D-glucopyranoside are correlated to pH of the medium， The stability of the new formulation of the drugs can be increased by changing the pH value. Its measurement results are important to further research to Piceatannol 3’-O-β-D-glucopyranoside， formulation research and in vivo absorption， distribution and metabolic processes important reference.